Publication - Abstract
Feb 15, 2021
Molecular Therapy Nucleic Acids
December 15, 2016
Multi-functional nanomaterials possess unique properties, facilitating both therapeutic and diagnostic applications among others. Herein, we developed dual-functional lipid-like nanoparticles for simultaneous delivery of mRNA and magnetic resonance imaging (MRI) contrast agents in order to express functional proteins and provide real-time visualization. TT3-Gd18 LLNs were identified as a lead formulation, which was able to encapsulate 91% of mRNA and 74% of Gd. This formulation showed a comparable or a slightly higher delivery efficiency of mRNA compared to the initial TT3 LLNs. Moreover, a strong MRI signal was observed in the cell pellets treated with TT3-Gd18 LLNs. More importantly, TT3-Gd18 LLNs demonstrated an efficient delivery of mRNA and Gd contrast agents in vivo.
Publication - Abstract
Feb 15, 2021
Molecular Therapy Nucleic Acids
Publication - Summary
Jun 19, 2016
Neuromethods
This protocol outlines a high-content screening (HCS) platform for investigating the impact of microRNA (miRNA) expression on primary motor neurons in culture. Such a system is applicable to automated screening of cell morphology in response to dru...