Expediting in Vitro Characterization of mRNA-Based Gene Therapies via High-Content Fluorescent Imaging


Authors: T.N. Vigila, D. Zhang-Hulsey, J.L. Santos and G.P. Hussmann

Journal: Analytical Biochemistry

DOI: https://doi.org/10.1016/j.ab.2021.114259

Publication - Abstract

August 15, 2021

mRNA Nucleic Acid Lipid Nanoparticles
A study conducted by Vigil et al. 2021 from Astrazeneca Biopharmaceuticals R&D showcases the applicability of high-content fluorescent imaging as a technique for the rapid characterization of mRNA-LNPs generated from a NanoAssemblr® mixing device in order to determine the optimal conditions for transfection that minimize cellular cytotoxicity and maximize mRNA internalization. Through high-content fluorescent imaging, mRNA-LNP characteristics such as transfection parameters, transfection-induced cytotoxicity, mRNA delivery, and mRNA translation were investigated in HEPG2 two dimensional monolayer and three dimensional spheroid cell cultures. This research demonstrates the value in utilizing high fluorescent imaging especially for future studies toward the development of novel gene therapies and drug candidates for various disease areas.

limit search to this category
Advanced Search

Advanced Search

close
  • Publications
  • Application Notes
  • Posters
  • Workshops
  • Videos & Webinars
  • Articles
Search

Browse by Category

  • Application
    • Diagnostic and Imaging
    • Genetic Medicine
    • Hematology
    • Metabolic Disorders
    • Neuroscience
    • Oncology
    • Skeletal Disorders
    • Targeted Drug Delivery
    • Vaccines
    • Other Applications
    • Cell therapy
  • Formulation
    • Liposomes
    • Nucleic Acid Lipid Nanoparticles
    • Polymeric Nanoparticles
    • Other Formulations
  • Payload
    • DNA
    • microRNA
    • mRNA
    • siRNA
    • Small Molecule Drugs
    • Other Payloads


related content

Publication - Abstract

Aug 26, 2016
Biochemical and Biophysical Research Communications

GLUT3 is Present in Clone 9 Liver Cells and Translocates to the Plasma Membrane in Response to Insulin

D.M. Defries, C.G. Taylor and P. Zahradka

Metabolic Disorders Nucleic Acid Lipid Nanoparticles siRNA

Clone 9 cells have been reported to express only the GLUT1 facilitative glucose transporter; however, previous studies have not examined Clone 9 cells for GLUT3 content. The current study sought to profile the presence of glucose transporters in Cl...

Read More


Publication - Abstract

May 13, 2021
Nature Communications

Tissue-specific Activation of Gene Expression by the Synergistic Activation Mediator (SAM) CRISPRa System in Mice

C. Hunt, S.A. Hartford, G. Gong, et al

mRNA Genetic Medicine Other Applications
The paper demonstrates PNI's mRNA-LNP as highly capable of inducing strong levels of upregulation in target genes with activation levels six to nine fold dependent on amount of gRNA packaged. The paper validates the SAM system in mice as a suitable in vivo model encompassing both...
Read More


Sign Up and Stay Informed
Sign up today to automatically receive new Precision NanoSystems application notes, conference posters, relevant science publications, and webinar invites.
Sign Up