Investigating the Impact of Delivery System Design on the Efficacy of Self-Amplifying RNA Vaccines


Authors: G. Anderluzzi, G. Lou, S. Gallorini, M. Brazzoli, R. Johnson, D.T. O'Hagan, B.C. Baudner and Y. Perrie

Journal: Vaccines

DOI: 10.3390/vaccines8020212

Publication - Abstract

May 08, 2020

mRNA Polymeric Nanoparticles Nucleic Acid Lipid Nanoparticles

Abstract

messenger RNA (mRNA)-based vaccines combine the positive attributes of both live-attenuated and subunit vaccines. In order for these to be applied for clinical use, they require to be formulated with delivery systems. However, there are limited in vivo studies which compare different delivery platforms. Therefore, we have compared four different cationic platforms: (1) liposomes, (2) solid lipid nanoparticles (SLNs), (3) polymeric nanoparticles (NPs) and (4) emulsions, to deliver a self-amplifying mRNA (SAM) vaccine. All formulations contained either the non-ionizable cationic lipid 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) or dimethyldioctadecylammonium bromide (DDA) and they were characterized in terms of physico-chemical attributes, in vitro transfection efficiency and in vivo vaccine potency. Our results showed that SAM encapsulating DOTAP polymeric nanoparticles, DOTAP liposomes and DDA liposomes induced the highest antigen expression in vitro and, from these, DOTAP polymeric nanoparticles were the most potent in triggering humoral and cellular immunity among candidates in vivo.

limit search to this category
Advanced Search

Advanced Search

close
  • Publications
  • Application Notes
  • Posters
  • Workshops
  • Videos & Webinars
  • Articles
Search

Browse by Category

  • Application
    • Diagnostic and Imaging
    • Genetic Medicine
    • Hematology
    • Metabolic Disorders
    • Neuroscience
    • Oncology
    • Skeletal Disorders
    • Targeted Drug Delivery
    • Vaccines
    • Other Applications
    • Cell therapy
  • Formulation
    • Liposomes
    • Nucleic Acid Lipid Nanoparticles
    • Polymeric Nanoparticles
    • Other Formulations
  • Payload
    • DNA
    • microRNA
    • mRNA
    • siRNA
    • Small Molecule Drugs
    • Other Payloads


related content

Publication - Summary

Apr 12, 2019
Journal of Controlled Release

Lipid Nanoparticles for Delivery of Messenger RNA to the Back of the Eye

S. Patel, R.C. Ryals, K.K.Weller, M.E. Pennesi and G. Sahay

mRNA Genetic Medicine Nucleic Acid Lipid Nanoparticles
Gaurav Sahay’s lab at Oregon Health and Science University describe a systematic study of lipid nanoparticles (LNPs) for delivering mRNA to the retina. Eleven formulations were made using NanoAssemblr technology and some were administered by subretinal injection in mice. Th...
Read More


Publication - Summary

May 11, 2017
Biomaterials

Selective Targeting and Therapy of Metastatic and Multidrug Resistant Tumors Using a Long Circulating Podophyllotoxin Nanoparticle

A. Roy, Y. Zhao, Y. Yang, A. Szeitz, T. Klassen, S.D. Li

Small Molecule Drugs Polymeric Nanoparticles Oncology

Metastatic and multidrug resistant (MDR) tumors are generally very challenging to treat; and while in many cancers early detection can increase the survival rate significantly, the mortality rates of advanced and metastasized cancers are still quit...

Read More


Sign Up and Stay Informed
Sign up today to automatically receive new Precision NanoSystems application notes, conference posters, relevant science publications, and webinar invites.
Sign Up