Effects of Local Structural Transformation of Lipid-Like Compounds on Delivery of Messenger RNA


Authors: B. Li, X. Luo, B. Deng, J.B. Giancola, D.W. McComb, T.D. Schmittgen and Y. Dong

Journal: Scientific Reports

DOI: 10.1038/srep22137

Publication - Abstract

February 26, 2016

mRNA Nucleic Acid Lipid Nanoparticles Genetic Medicine

Abstract:

Lipid-like nanoparticles (LLNs) have shown great potential for RNA delivery. Lipid-like compounds are key components in LLNs. In this study, we investigated the effects of local structural transformation of lipid-like compounds on delivery of messenger RNA. Our results showed that position change of functional groups on lipid-like compounds can dramatically improve delivery efficiency. We then optimized formulation ratios of TNT-b10 LLNs, a lead material, increasing delivery efficiency over 2-fold. More importantly, pegylated TNT-b10 LLNs is stable for over four weeks and is over 10-fold more efficient than that of its counterpart TNT-a10 LLNs. Additionally, the optimal formulation O-TNT-b10 LLNs is capable of delivering mRNA encoding luciferase in vivo. These results provide useful insights into the design of next generation LLNs for mRNA delivery.


limit search to this category
Advanced Search

Advanced Search

close
  • Publications
  • Application Notes
  • Posters
  • Workshops
  • Videos & Webinars
  • Articles
Search

Browse by Category

  • Application
    • Diagnostic and Imaging
    • Genetic Medicine
    • Hematology
    • Metabolic Disorders
    • Neuroscience
    • Oncology
    • Skeletal Disorders
    • Targeted Drug Delivery
    • Vaccines
    • Other Applications
    • Cell therapy
  • Formulation
    • Liposomes
    • Nucleic Acid Lipid Nanoparticles
    • Polymeric Nanoparticles
    • Other Formulations
  • Payload
    • DNA
    • microRNA
    • mRNA
    • siRNA
    • Small Molecule Drugs
    • Other Payloads


related content

Publication - Abstract

Jan 28, 2021
Hepatology

Therapeutic Shutdown of HBV Transcripts Promotes Reappearance of the SMC5/6 Complex and Silencing of the Viral genome In Vivo

L. Allweiss, K. Giersch, A. Pirosu, T. Volz, R.C. Muench, R.K. Beran, S. Urban, H. Javanbakht, S.P. Fletcher, M. Lütgehetmann and M. Dandri

siRNA Hematology Other Applications
These results reveal that therapeutics abrogating all HBV transcripts including HBx promote epigenetic suppression of the HBV minichromosome, whereas strategies protecting the human hepatocytes from reinfection are needed to maintain cccDNA silencing.
Read More


Publication - Abstract

Dec 15, 2016
Nanoscale

Dual-Functional Lipid-Like Nanoparticles for Delivery of mRNA and MRI Contrast Agents

X. Luo, B. Li, X. Zhang, W. Zhao, A. Bratasz, B. Deng, D.W. McComb and Y. Dong

Diagnostic and Imaging Nucleic Acid Lipid Nanoparticles mRNA

Multi-functional nanomaterials possess unique properties, facilitating both therapeutic and diagnostic applications among others. Herein, we developed dual-functional lipid-like nanoparticles for simultaneous delivery of mRNA and magnetic resonance...

Read More


Sign Up and Stay Informed
Sign up today to automatically receive new Precision NanoSystems application notes, conference posters, relevant science publications, and webinar invites.
Sign Up