Search Results - Resource Center

PUBLICATIONS

Publication - Abstract

Apr 20, 2021
ACS Biomaterials Science & Engineering

Manual Versus Microfluidic-Assisted Nanoparticle Manufacture: Impact of Silk Fibroin Stock on Nanoparticle Characteristics

J.I. Solomun, J.D. Totten, T. Wongpinyochit, A.J. Florence and F.P. Seib

Other Payloads Other Formulations Other Applications

Silk has a long track record of clinical use in the human body, and new formulations, including silk nanoparticles, continue to reveal the promise of this natural biopolymer for healthcare applications. Native silk fibroin can be isolated directly from the silk gland, but generat...
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Publication - Abstract

Mar 18, 2021
Molecular Therapy

Bioinspired Artificial Exosomes Based on Lipid Nanoparticles Carrying let-7b-5p Promote Angiogenesis In Vitro and In Vivo

S. Aday, I. Hazan-Helevy, A. Chamorro-Joganes, M. Anwar, D. Peer, C. Emanueli, et. al.

mRNA Other Formulations Hematology

MicroRNAs (miRNAs) regulate gene expression by post-transcriptional inhibition of target genes. Proangiogenic small extracellular vesicles (sEVs; popularly identified with the name “exosomes”) with a composite cargo of miRNAs are secreted by cultured stem cells and pr...
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Publication - Abstract

Mar 15, 2021
Acta Biomaterilia

Nanoparticles for Delivery of Agents to Fetal Lungs

S.J. Ullrich, M. Freedman-Weiss, S. Ahle, D.H. Stitelman, et. al.

Small Molecule Drugs Targeted Drug Delivery Polymeric Nanoparticles

Fetal treatment of congenital lung disease, such as cystic fibrosis, Read More
Publication - Abstract

Mar 12, 2021
Pharmaceutics

LipoParticles: Lipid-Coated PLA Nanoparticles Enhanced In Vitro mRNA Transfection Compared to Liposomes

C. Ayad, P. Libeau, C. Lacroix-Gimon, C. Ladaviére and B. Verrier

mRNA Genetic Medicine DNA

The approval of two mRNA vaccines as urgent prophylactic treatments against Covid-19 made them a realistic alternative to conventional vaccination methods. However, naked mRNA is rapidly degraded by the body and cannot effectively penetrate cells. Vectors capable of addressing th...
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Publication - Abstract

Feb 25, 2021
Molecular Cancer

mRNA Vaccine for Cancer Immunotherapy

L. Miao, Y. Zhang and L. Huang

mRNA Nucleic Acid Lipid Nanoparticles Other Formulations

mRNA vaccines have become a promising platform for cancer immunotherapy. During vaccination, naked or vehicle loaded mRNA vaccines efficiently express tumor antigens in antigen-presenting cells (APCs), facilitate APC activation and innate/adaptive immune stimulation. mRNA cancer ...
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Publication - Abstract

Feb 16, 2021
Advanced Materials

A Comprehensive Review on Intracellular Delivery

D.M. Rad, M.A. Rad, S.R. Bazaz, N. Kashaninejad, D. Jin and M.E. Warkiani

Other Payloads Other Formulations Other Applications

Intracellular delivery is considered an indispensable process for various studies, ranging from medical applications (cell‐based therapy) to fundamental (genome‐editing) and industrial (biomanufacture) approaches. Conventional macroscale delivery systems critically suffer from su...
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Publication - Abstract

Jan 08, 2021
Scientific Reports

Comparing Microfluidics and Ultrasonication as Formulation Methods for Developing Hempseed Oil Nanoemulsions for Oral Delivery Applications

F. Fathordoobady, N. Sannikova, Y. Guo, A. Singh, D.D. Kitts and A. Pratap-Singh

Small Molecule Drugs Other Formulations Other Applications

Emerging formulation technologies aimed to produce nanoemulsions with improved characteristics, such as stability are attractive endeavors; however, comparisons between competing technologies are lacking. In this study, two formulation techniques that employed ultrasound and micr...
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Publication - Abstract

Nov 26, 2020
Journal of Controlled Release

Effect of Complexing Lipids on Cellular Uptake and Expression of Messenger RNA in Human Skin Explants

A.K.. Blakney, P. Deletic, P.F. McKay, C.R. Bouton, M. Ashford, R.J. Shattock and A. Sabirsh

Genetic Medicine mRNA Vaccines

mRNA was formulated in lipoplexes with ionizable, cationic or zwitterionic complexing lipids.

Cell populations in human skin were analyzed for RNA uptake and expression over 72 h.

Adipocytes exhibit...
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Publication - Abstract

Nov 26, 2020
European Journal of Pharmaceutics and Biopharmaceutics

Evaluation of Bovine Milk Extracellular Vesicles for the Delivery of Locked Nucleic Acid Antisense Oligonucleotides

P. Grossen, M. Portmann, E. Koller, M. Duschmalé, T. Minz, S. Sewing and M. Keller et. al.

siRNA Other Applications Other Formulations

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Publication - Abstract

Nov 20, 2020
nanomaterials

Biomimetic Nanoparticles Potentiate the Anti-Inflammatory Properties of Dexamethasone and Reduce the Cytokine Storm Syndrome: An Additional Weapon against COVID-19?

R. Molinaro, A. Pasto, F. Taraballi, F. Giordano, J.A. Azzi, E. Tasciotti and C. Corbo

Small Molecule Drugs Other Formulations Other Applications

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Publication - Abstract

Nov 09, 2020
pharmaceutics

The Importance of Poly(ethylene glycol) and Lipid Structure in Targeted Gene Delivery to Lymph Nodes by Lipid Nanoparticles

D. Zukancic, E.J.A. Suys, E.H. Pilkington, A. Algarni, H. Al-Wassiti and N.P. Truong

DNA Nucleic Acid Lipid Nanoparticles Vaccines

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Publication - Abstract

Nov 04, 2020
Science Advances

CD8+ T Cells Mediate Protection Against Zika Virus Induced by an NS3-based Vaccine

A.E. Ngono, T. Syed, A.V. Nguyen, J.A. Regla-Nava, M. Susantono, D. Spasova, S. Shresta et. al.

Other Payloads Other Formulations Vaccines

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Publication - Abstract

Nov 02, 2020
Journal of Controlled Release

Rational Design of Adjuvants for Subunit Vaccines: The Format of Cationic Adjuvants Affects the Induction of Antigen-specific Antibody Responses

G. Anderluzzi, S.T. Schmidt, R. Cunliffe, S. Woods, C.W. Roberts, D. Veggi, I. Ferlenghi, D.T. O'Hagan, B.C. Baudner and Y. Perrie

Other Payloads Other Formulations Polymeric Nanoparticles

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Publication - Abstract

Oct 06, 2020
ACS Applied Nano Materials

Versatile and High-Throughput Strategy for the Quantification of Proteins Bound to Nanoparticles

R. Oliverio, B. Liberelle, F. Murschel, A. Garcia-Ac, X. Banquy and G. De Crescenzo

Polymeric Nanoparticles Other Applications Other Formulations

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Publication - Abstract

Sep 25, 2020
ACS Applied Nano Materials

Polysarcosine-Functionalized Lipid Nanoparticles for Therapeutic mRNA Delivery

S.S. Nogueira, A. Schlegel, K. Maxeiner, B. Weber, M. Barz, H. Haas et. al.

mRNA Nucleic Acid Lipid Nanoparticles Other Applications

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Publication - Abstract

Sep 10, 2020
Drug Delivery Letters

Microfluidic Manufacture of Solid Lipid Nanoparticles: A Case Study on Tristearin-Based Systems

G. Anderluzzi and Y. Perrie

Other Applications Other Payloads Other Formulations

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Publication - Abstract

Sep 01, 2020
International Journal of Pharmaceutics

Microfluidic Preparation of Various Perfluorocarbon Nanodroplets: Characterization and Determination of Acoustic Droplet Vaporization (ADV) Threshold

R. Melich, P. Bussat, L. Morici, A. Vivien, E. Gaud, T. Bettinger and S. Cherkaoui

Other Payloads Other Formulations Other Applications

Over the last two decades, liquid perfluorocarbon nanodroplets (PFC-NDs), also known as Phase Change Contrast Agents (PCCAs), that are capable of vaporizing into gaseous echogenic microbubbles via an external stimulus, have gained much attention for diagnostic and therapeutic app...
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Publication - Abstract

Aug 21, 2020
Science Advances

Functionalized Lipid-like Nanoparticles for In Vivo mRNA Delivery and Base Editing

X. Zhang, W. Zhao, G.N. Nguyen, C. Zhang, Y. Dong et. al.

mRNA Hematology Other Formulations

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Publication - Abstract

Jul 01, 2020
Journal of Controlled Release

Delivery of Self-amplifying mRNA Vaccines by Cationic Lipid Nanoparticles: The Impact of Cationic Lipid Selection

G. Lou, G. Anderluzzi, S.T. Schmidt, S. Woods, S. Gallorini, M. Brazzoli, F. Giusti, I. Ferlenghi, R. Johnson, C.W. Roberts, D.T. O'Hagan, B.C. Baudner and Y. Perrie

Other Applications Nucleic Acid Lipid Nanoparticles mRNA

Self-amplifying RNA (SAM) represents a versatile tool that can be used to develop potent vaccines, potentially able to elicit strong antigen-specific humoral and cellular-mediated immune responses to virtually any infectious disease. To protect the SAM from degradation and achiev...
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Publication - Abstract

Jun 22, 2020
Small

Shedding Light on Metal‐Based Nanoparticles in Zebrafish by Computed Tomography with Micrometer Resolution

E. Cörek, G. Rodgers, S. Siegrist, T. Einfalt, P. Detampel, C.M. Schlepütz, S. Sieber, P. Fluder, G. Schulz, H. Unterweger, C. Alexiou, B. Müller, M. Puchkov and J. Huwyler

Other Formulations Other Payloads Other Applications

Metal‐based nanoparticles are clinically used for diagnostic and therapeutic applications. After parenteral administration, they will distribute throughout different organs. Quantification of their distribution within tissues in the 3D space, however, remains a challenge owing to...
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Publication - Abstract

Jun 20, 2020
Advanced Drug Delivery Reviews

Lipid-based Nanoparticle Technologies for Liver Targeting

R. Böttger, G. Pauli, P. Chao, N.A.L. Fayez, L. Hohenwarter and S.D. Li

siRNA Other Formulations mRNA

Liver diseases such as hepatitis, cirrhosis, and hepatocellular carcinoma are global health problems accounting for approximately 800 million cases and over 2 million deaths per year worldwide. Major drawbacks of standard pharmacological therapies are the inability to deliver a s...
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Publication - Abstract

Jun 15, 2020
Chemical Engineering Journal

Microfluidic Synthesis Control Technology and Its Application in Drug Delivery, Bioimaging, Biosensing, Environmental Analysis and Cell Analysis

C.T. Kung, H. Gao, C.Y. Lee, Y.N. Wang, W. Dong C.H. Ko, G. Wang and L.M. Fu

Other Formulations Liposomes Other Applications

Nanomaterials have attracted significant attention in biomedical and environmental science in recent years due to their unique thermal, optical, electrical and magnetic properties. The properties of nanomaterials are highly correlated with their size and morphology. Consequently,...
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Publication - Abstract

Jun 05, 2020
Journal of Controlled Release

Glucosamine-NISV Delivers Antibody Across the Blood-brain Barrier: Optimization for Treatment of Encephalitic Viruses

S. Woods, L.M. O'Brien, W. Butcher, J.E. Preston, A.R. Georgian, E.D. Williamson, F.J. Salguero, F. Modino, N.J. Abbott, C.W. Roberts and R.V. D'Elia

Other Formulations Other Payloads Vaccines

The field of brain drug delivery faces many challenges that hinder development and testing of novel therapies for clinically important central nervous system disorders. Chief among them is how to deliver large biologics across the highly restrictive blood-brain barrier. Non-ionic...
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Publication - Abstract

Jun 01, 2020
MethodsX

Synthesis of Protein Conjugates Adsorbed on Cationic Liposomes Surface

D. Chatzikleanthous, R. Cunliffe, F. Carboni, M.R. Romano, D.T. O'Hagan, C.W. Roberts, Y. Perrie and R. Adamo

Vaccines Other Payloads Liposomes

The well-known Toll like receptor 9 (TLR9) agonist CpG ODN has shown promising results as vaccine adjuvant in preclinical and clinical studies, however its in vivo stability and potential systemic toxicity remain a concern. In an effort to overcome these issues...
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Publication - Abstract

May 28, 2020
Journal of Liposome Research

Examination of the Effect of Niosome Preparation Methods in Encapsulating Model Antigens on the Vesicle Characteristics and Their Ability to Induce Immune Responses

M.A. Obeid, T. Teeravatcharoenchai, D. Connell, K. Niwasabutra, M. Hussain, K. Carter and V.A. Ferro

Vaccines Other Formulations Other Payloads

Niosome nanoparticles can be prepared using different methods, each of which can affect the size and homogeneity of the prepared particles. The aim of this study was to establish if the method of preparation impacted on the prepared vesicles when loaded with a model protein and t...
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Publication - Abstract

May 04, 2020
European Journal of Pharmaceutics and Biopharmaceutics

Lipid-encapsulated Oral Therapeutic Peptide Vaccines Reduce Tumour Growth in an Orthotopic Mouse Model of Colorectal Cancer

M. Naciute, V. Niemi, R.A. Kemp and S. Hook

Other Payloads Liposomes Oncology

The aim of this study was to develop an oral vaccine that could be used to treat colorectal cancer. Oral vaccines are technically challenging to develop due to the harsh gastric environment but have numerous benefits including high patient acceptability and the potential to stimu...
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Publication - Abstract

May 02, 2020
Processes

Comprehensive Screening of Drug Encapsulation and Co-Encapsulation into Niosomes Produced Using a Microfluidic Device

S. Joshi, R. White, R. Sahu, V.A. Dennis and S.R. Singh

Small Molecule Drugs Other Formulations

Microfluidics is a very facile and fast method of particulate production. Besides, it enables the manufacturing of size tuned particulate systems. Niosomes due to structural similarities have importance as alternative drug delivery systems to liposomes. Niosomes can be encapsulat...
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Publication - Abstract

Apr 15, 2020
International Journal of Pharmaceutics

Antisolvent Precipitation of Lipid Nanoparticles in Microfluidic Systems – A Comparative Study

J. Riewe, P. Erfle, S. Melzig, A. Kwade, A. Dietzel, and H. Bunjes

Other Formulations Liposomes

Microsystems offer promising possibilities to produce nanoparticles which can be used as carriers for poorly water-soluble active substances. The aim of the present study was to compare the preparation of lipid nanoparticles by precipitation in different microsystems: A segmented...
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Publication - Abstract

Mar 10, 2020
ACS Chemical Biology

Engineering Caveolae-Targeted Lipid Nanoparticles To Deliver mRNA to the Lungs

Q. Li, C. Chan, N. Peterson, R.N. Hanna, A. Alfaro, K.L. Allen, H. Wu, W.F. Dall'Acqua, M.J. Borrok and J.L. Santos

mRNA Nucleic Acid Lipid Nanoparticles Targeted Drug Delivery

Efficacious use of therapeutic gene delivery via nanoparticles is hampered by the challenges associated with targeted delivery to tissues of interest. Systemic administration of lipid nanoparticle (LNP)-encapsulated mRNA leads to a protein expressed predominantly in the liver and...
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Publication - Abstract

Feb 29, 2020
Biomaterials

Nose-to-brain Delivery of Enveloped RNA - Cell Permeating Peptide Nanocomplexes for the Treatment of Neurodegenerative Diseases

E. Samaridou, H. Walgrave, E. Salta, D.M. Álvarez, V. Castro-López, M. Loza, and M.J. Alonso

Other Formulations siRNA Other Applications

Direct nose-to-brain (N-to-B) delivery enables the rapid transport of drugs to the brain, while minimizing systemic exposure. The objective of this work was to engineer a nanocarrier intended to enhance N-to-B delivery of RNA and to explore its potential utility for the treatment...
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Publication - Abstract

Feb 20, 2020
Nanotechnology

Niosome-encapsulated Balanocarpol: Compound Isolation, Characterisation, and Cytotoxicity Evaluation Against Human Breast and Ovarian Cancer Cell Lines

M.A. Obeid, S.A.S. Gany, A.I. Gray, L. Young, J.O. Igoli and V.A. Ferro

Small Molecule Drugs Other Formulations Oncology

Natural products have been successfully used to treat various ailments since ancient times and currently several anticancer agents based on natural products are used as the main therapy to treat cancer patients, or as a complimentary treatment to chemotherapy or radiation. Balano...
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Publication - Abstract

Feb 10, 2020
Drug Delivery and Translational Research

Technological Challenges in the Preclinical Development of an HIV Nanovaccine Candidate

T.G. Dacoba, L. Ruiz-Gatón, A. Benito. M. Klein, D. Dupin, M. Luo, M. Menta, D. Teijeiro-Osorio, I. Loinaz, M.J. Alonso and J. Crecente-Campo

Polymeric Nanoparticles Other Payloads Vaccines

Despite a very active research in the field of nanomedicine, only a few nano-based drug delivery systems have reached the market. The “death valley” between research and commercialization has been partially attributed to the limited characterization and reproducibilit...
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Publication - Abstract

Jan 30, 2020
Pharmaceutics

Effect of Protein Corona on The Transfection Efficiency of Lipid-Coated Graphene Oxide-Based Cell Transfection Reagents

E. Quagliarini, R. Di Santo, S. Palchetti, G. Ferri, F. Cardarelli, D. Pozzi and G. Caracciolo

DNA Genetic Medicine Other Formulations

Coating graphene oxide nanoflakes with cationic lipids leads to highly homogeneous nanoparticles (GOCL NPs) with optimised physicochemical properties for gene delivery applications. In view of in vivo applications, here we use dynamic light scattering, micro-electrophoresis and o...
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Publication - Abstract

Oct 24, 2019
Circulation Research

Rapamycin-Loaded Biomimetic Nanoparticles Reverse Vascular Inflammation

C. Boada, A. Zinger, C. Tsao, P. Zhao, J.O. Martinez, K. Hartman, T. Naoi, R. Sukhoveshin, M. Sushnitha, R. Molinaro, B. Trachtenberg, J.P. Cooke, and E. Tasciotti

Other Formulations Other Payloads Other Applications

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Publication - Abstract

Sep 22, 2019
International Journal of Molecular Sciences

Rapid Microfluidic Preparation of Niosomes for Targeted Drug Delivery

D.A. Seleci, V. Maurer, F. Stahl, T. Scheper, and G. Garnweitner

Oncology Other Formulations Small Molecule Drugs

Niosomes are non-ionic surfactant-based vesicles with high promise for drug delivery applications. They can be rapidly prepared via microfluidics, allowing their reproducible production without the need of a subsequent size reduction step, by controlled mixing of two miscible pha...
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Publication - Abstract

Sep 17, 2019
Expert Opinion on Drug Delivery

Lipid-based Nanoparticle Formulations for Small Molecules and RNA Drugs

L.M. Ickenstein and P. Garidel

Other Formulations Nucleic Acid Lipid Nanoparticles

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Publication - Abstract

Jun 17, 2019
Lab on a Chip

The Biomolecular Corona of Gold Nanoparticles in a Controlled Microfluidic Environment

L. Digiacomo, S. Palchetti, F. Giulimondi, D. Pozzi, R.Z. Chiozzi, A.L. Capriotti, A. Laganà, and G. Caracciolo

Other Applications Other Formulations Other Payloads

Nanoparticles (NPs) exposed to biological media are coated by proteins and other biomolecules forming a biomolecular corona (BC) on the particle surface. Recent studies have shown that shear stress as that created by laminar fluid flow generates more complex coronas with systemat...
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Publication - Abstract

Jun 11, 2019
Applied Physics Letter

Microfluidic-generated Lipid-graphene Oxide Nanoparticles for Gene Delivery

R. Di Santo, E. Quagliarini, S. Palchetti, D. Pozzi, V. Palmieri, G. Perini, M. Papi, A.L. Capriotti, A. Laganà, and G. Caracciolo

DNA Other Formulations

Graphene oxide (GO) is employed in a broad range of biomedical applications including antimicrobial therapies, scaffolds for tissue engineering, and drug delivery, among others. However, the inability to load it efficiently with double-stranded DNA impairs its use as a gene deliv...
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Publication - Abstract

Jun 03, 2019
Journal of Controlled Release

Formulation and Manufacturing of Lymphatic Targeting Liposomes Using Microfluidics

S. Khakde, C.B. Roces, A. Cameron, A Devit and Y. Perrie

Oncology Vaccines Liposomes

The lymphatics are a target for a range of therapeutic purposes, including cancer therapy and vaccination and both vesicle size and charge have been considered as factors controlling lymphatic targeting. Within this work, a range...
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Publication - Abstract

May 15, 2019
Procceses

Using a Microfluidics System to Reproducibly Synthesize Protein Nanoparticles: Factors Contributing to Size, Homogeneity, and Stability

C. Ballegooie, A. Man, I. Andreu, B.D. Gates, and D. Yapp

Other Formulations

The synthesis of Zein nanoparticles (NPs) using conventional methods, such as emulsion solvent diffusion and emulsion solvent evaporation, is often unreliable in replicating particle size and polydispersity between batch-to-batch syntheses. We have systematically examined the par...
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Publication - Abstract

Feb 15, 2019
International Journal of Nanomedicine

Targeted Lipid Nanoparticle Delivery of Calcitriol to Human Monocyte-derived Macrophages In Vitro and In Vivo: Investigation of the Anti-inflammatory Effects of Calcitriol

A. Rafique, A. Etzerodt, J.H. Graversen, S.K. Moestrup, F. Dagnæs-Hansen and H.J.Møller

Small Molecule Drugs Other Formulations Other Applications

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Publication - Summary

Feb 07, 2019
Nano Research

Chemotherapy Drugs Derived Nanoparticles Encapsulating mRNA Encoding Tumor Suppressor Proteins to Treat Triple-negative Breast Cancer

C. Zhang, X. Zhang, W. Zhao, C. Zeng, W. Li, B. Li, X. Luo, J. Li, J. Jiang, B. Deng, D.W. McComb and Y. Dong

mRNA Nucleic Acid Lipid Nanoparticles Oncology

Yizhou Dong’s group at Ohio State University have published a paper describing a small molecule/mRNA lipid nanoparticle combination therapy for triple negative breast cancer (TNBC). TNBC represents about 15% of all breast cancers. The Dong lab’s approach involved deli...
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Publication - Abstract

Jan 29, 2019
Pharmaceutics

Advances of Non-Ionic Surfactant Vesicles (Niosomes) and Their Application in Drug Delivery

X. Ge, M. Wei, S. He and W-E. Yuan

Small Molecule Drugs Other Formulations Other Payloads

Non-Ionic surfactant based vesicles, also known as niosomes, have attracted much attention in pharmaceutical fields due to their excellent behavior in encapsulating both hydrophilic and hydrophobic agents. In recent years, it has been discovered that these vesicles can improve th...
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Publication - Summary

Jan 17, 2019
Nanomedicine: Nanotechnology, Biology and MEdicine

Zebrafish as a Predictive Screening Model to Assess Macrophage Clearance of Liposomes In Vivo

S. Sieber, P. Grossen, P. Uhl, P. Detampel, W. Mier, D. Witzigmann, J. Huwyler

Liposomes

The Witzigmann and Huwyler labs at the University of Basel examined the in vivo clearance of previously approved liposome formulations in zebrafish embryos and rats to demonstrate that zebrafish embryos provide similar results while being are a cheaper and faster to stud...
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Publication - Summary

Jan 06, 2019
Nanoscale

Microfluidic manufacturing of surface-functionalized graphene oxide nanoflakes for gene delivery

R. Di Santo, L. Digiacomo, S. Palchetti, V. Palmieri, G. Perini, D. Pozzi, M. Papi and G. Caracciolo

DNA Other Formulations Genetic Medicine

Scientists at Sapienza University in Rome report using the NanoAssemblr Benchtop to make Graphene oxide/Cationic lipid (GOCL) hybrid particles for gene delivery. They compared plasmid delivery to standard DOTAP lipoplexes in HEK and HeLa cells and found that transfection efficien...
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Publication - Abstract

Dec 09, 2018
Pharmaceutics

The Microfluidic Technique and the Manufacturing of Polysaccharide Nanoparticles

E. Chiesa, R. Dorati, S. Pisani, B. Conti, G. Bergamini, T. Modena and I. Genta

Other Formulations Other Applications Small Molecule Drugs

The microfluidic technique has emerged as a promising tool to accelerate the clinical translation of nanoparticles, and its application affects several aspects, such as the production of nanoparticles and the in vitro characterization in the microenvironment, mimicking <...
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Publication - Abstract

Nov 30, 2018
Nanoscale Advances

Microfluidic-assisted Silk Nanoparticle Tuning

T. Wongpinyochit, J.D. Totten, B.F. Johnston and F. Philipp Seib

Other Applications Other Formulations

Silk is now making inroads into advanced pharmaceutical and biomedical applications. Both bottom-up and top-down approaches can be applied to silk and the resulting aqueous silk solution can be processed into a range of material formats, including nanoparticles. Here, we demonstr...
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Publication - Abstract

Nov 29, 2018
Carbohydrate Polymers

N-oxy Lipid-based Click Chemistry for Orthogonal Coupling of Mannan onto Nanoliposomes Prepared by Microfluidic Mixing...

E. Bartheldyová, P.T. Knotigová, K. Zachová and J. Turánek et al.

Vaccines Liposomes Other Payloads

New synthetic aminooxy lipid was designed and synthesized as a building block for the formulation of functionalised nanoliposomes (presenting onto the outer surface of aminooxy groups) by microfluidic mixing. Orthogonal binding of cellular mannan (Candida glabrata (CCY 26-20-1) o...
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Publication - Abstract

Nov 29, 2018
International Journal of Pharmaceutics

Rapid and Scale-independent Microfluidic Manufacture of Liposomes Entrapping Protein Incorporating In-line Purification and At-line Size Monitoring

N. Forbes, MT. Hussain, ML. Briuglia, DY. Edwards, JH. terHorst, N. Szita, Y. Perrie

Other Payloads Other Applications Liposomes

Within this paper we present work that has the ability to de-risk the translation of liposomes from bench to the clinic. We have used microfluidics for the rapid and scale-independent manufacture of liposomes and have incorporated in-line purification and at-line monitoring of pa...
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Publication - Abstract

Nov 12, 2018
Biomaterials Science

Degradable Redox-responsive Disulfide-based Nanogel Drug Carriers via Dithiol Oxidation Polymerization

S.A. Elkassih, P. Kos, H. Xiong and D.J. Siegwart

Small Molecule Drugs Other Formulations Other Applications

Stimuli-responsive nanogels are important drug and gene carriers that mediate the controlled release of therapeutic molecules. Herein, we report the synthesis of fully degradable disulfide cross-linked nanogel drug carriers formed by oxidative radical polymerization of 2,2′...
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Publication - Abstract

Oct 10, 2018
Drug Delivery and Translational Research

Rapid Optimization of Liposome Characteristics Using a Combined Microfluidics and Design-of-experiment Approach

M. Sedighi, S. Sieber, F. Rahimi, M.A. Shahbazi, A.H. Rezayan, J. Huwyler and D. Witzigmann

Liposomes Small Molecule Drugs

Liposomes have attracted much attention as the first nanoformulations entering the clinic. The optimization of physicochemical properties of liposomes during nanomedicine development however is time-consuming and challenging despite great advances in formulation development. Here...
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Publication - Abstract

Oct 01, 2018
Cellular and Molecular Bioengineering

Lipid Polymer Hybrid Nanomaterials for mRNA Delivery

W. Zhao, C. Zhang, B. Li, X. Zhang, X. Luo, C. Zeng, W. Li, M. Gao and Y. Dong

mRNA Genetic Medicine Other Formulations

Introduction: In the past decade, messenger RNA (mRNA) has been extensively explored in a wide variety of biomedical applications. However, efficient delivery of mRNA is still one of the key challenges for its broad applications in the clinic. Recently, lipid polymer hybrid nanop...
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Publication - Abstract

Sep 17, 2018
Bioengineering and Translational Medicine

Engineering, On‐demand Manufacturing, and Scaling‐up of Polymeric Nanocapsules

J. Crecente-Campo and M.J. Alonso

Other Formulations Other Applications

Polymeric nanocapsules are versatile delivery systems with the capacity to load lipophilic drugs in their oily nucleus and hydrophilic drugs in their polymeric shell. The objective of this work was to expand the technological possibilities to prepare customized nanocapsules. Firs...
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Publication - Abstract

Sep 05, 2018
Nanotheranostics

A Scalable Method for Squalenoylation and Assembly of Multifunctional 64Cu-Labeled Squalenoylated Gemcitabine Nanoparticles

S.T. Tucci, J.W. Seo, H. Kakwere, A. Kheirolomoom, E.S. Ingham, L.M. Mahakian, S. Tam, S. Tumbale, M. Baikoghli, H. Cheng and K.W. Ferrara

Other Formulations Small Molecule Drugs Oncology

Squalenoylation of gemcitabine, a front-line therapy for pancreatic cancer, allows for improved cellular-level and system-wide drug delivery. The established methods to conjugate squalene to gemcitabine and to form nanoparticles (NPs) with the squalenoylated gemcitabine (SqGem) c...
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Publication - Abstract

Jul 04, 2018
Nature communications

Rapid Transport of Deformation-tuned Nanoparticles Across Biological Hydrogels and Cellular Barriers

M. Yu, L. Xu, F. Tian, Q. Su, N. Zheng and Y. Yang

Small Molecule Drugs Other Formulations Polymeric Nanoparticles

To optimally penetrate biological hydrogels such as mucus and the tumor interstitial matrix, nanoparticles (NPs) require physicochemical properties that would typically preclude cellular uptake, resulting in inefficient drug delivery.
Read More
Publication - Abstract

Apr 05, 2018
Advanced Drug Delivery Reviews

Transforming Nanomedicine Manufacturing Toward Quality by Design and Microfluidics

S. Colombo, M. Beck-Broichsitter, J.P. Bøtker, M. Malmsten, J. Rantanen and A. Bohr

Small Molecule Drugs Polymeric Nanoparticles Other Formulations

Nanopharmaceuticals aim at translating the unique features of nano-scale materials into therapeutic products and consequently their development relies critically on the progression in manufacturing technology to allow scalable processes complying w...
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Publication - Abstract

Mar 19, 2018
Advanced Drug Delivery Reviews

Advances in Microfluidics for Lipid Nanoparticles and Extracellular Vesicles and Applications in Drug Delivery Systems

M. Maekia, N. Kimurab, Y. Satoc, H. Harashimac and M. Tokeshi

Small Molecule Drugs mRNA DNA

Lipid-based nanobiomaterials as liposomes and lipid nanoparticles (LNPs) are the most widely used nanocarriers for drug delivery systems (DDSs). Extracellular vesicles (EVs) and exosomes are also expected to be applied as DDS nanocarriers. The perf...
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Publication - Abstract

Feb 12, 2018
Journal of Nanobiotechnology

Optimization and Scale up of Microfluidic Nanolipomer Production Method for Preclinical and Potential Clinical Trials

A. Gdowski, K. Johnson, S. Shah, I. Gryczynski, J. Vishwanatha and A. Ranjan

Small Molecule Drugs Polymeric Nanoparticles Other Formulations

The process of optimization and fabrication of nanoparticle synthesis for preclinical studies can be challenging and time consuming. Traditional small scale laboratory synthesis techniques suffer from batch to batch variability. Additionally, the p...
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Publication - Abstract

Jun 03, 2017
Molecular Pharmaceutics

Formulation of Non-Ionic Surfactant Vesicles (NISV) Prepared by Microfluidics for Therapeutic Delivery of siRNA into Cancer Cells

M.A. Obeid, A. Elburi, L.C. Young, A.B. Mullen, R.J. Tate and V.A. Ferro

siRNA Other Formulations Oncology

Small interfering RNAs (siRNA) have a broad potential as therapeutic agents to reversibly silence any target gene of interest. The clinical application of siRNA requires the use of safe and effective delivery systems. In this study, we investigated...
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Publication - Abstract

May 27, 2017
Journal of Pharmaceutical Sciences

Self-Assembled Core-Shell-Type Lipid-Polymer Hybrid Nanoparticles: Intracellular Trafficking and Relevance for Oral Absorption

Q. Li, D. Xia , J. Tao, A. Shen, Y. He, Y. Gan and C. Wang

Small Molecule Drugs Other Formulations Other Applications

Lipid-polymer hybrid nanoparticles (NPs) are advantageous for drug delivery. However, their intracellular trafficking mechanism and relevance for oral drug absorption are poorly understood. In this study, self-assembled core-shell lipid-polymer hyb...
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Publication - Abstract

Apr 20, 2017
International Journal of Pharmaceutics

Comparison of the Physical Characteristics of Monodisperse Non-Ionic Surfactant Vesicles (NISV) Prepared Using Different Manufacturing Methods

M. Obeid, A. Gebril, R. Tate, A. Mullen and V. Ferro

Other Formulations

Non-ionic surfactant vesicles (NISV) are synthetic membrane vesicles formed by self-assembly of a non-ionic surfactant, often in a mixture with cholesterol and a charged chemical species. Different methods can be used to manufacture NISV, with the majority of these requiring bulk...
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Publication - Abstract

Nov 21, 2016
Nanoscale

Cationic Switchable Lipids: pH-triggered Molecular Switch for siRNA Delivery

W. Viricel, S. Poirier, A. Mbarek, R.M. Derbali, G. Mayer and J. Leblond

Other Formulations Nucleic Acid Lipid Nanoparticles siRNA

A pH-sensitive molecular switch able to change its conformation upon protonation at endosomal pH values is embedded into the structure of cationic lipidoid materials, thus conferring endosomal escape properties. Involvement of the conformational switch in the endosomal escape pro...
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Publication - Abstract

Nov 09, 2016
International Journal of Pharmaceutics

The Effects of Hydration Media on the Characteristics of Non-Ionic Surfactant Vesicles (NISV) Prepared by Microfluidics

M. Obeid, I. Khadraa, A. Mullena, R. Tatea and V. Ferroa

Other Formulations

Non-ionic surfactant vesicles (NISV) are colloidal particles that provide a useful delivery system for drugs and vaccines. One of the methods that is used for NISV preparation is microfluidics in which the lipid components dissolved in organic phas...
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Publication - Abstract

Nov 02, 2016
Journal of Pharmaceutical Sciences

Spontaneous Self-Assembly of Polymeric Nanoparticles in Aqueous Media: New Insights From Microfluidics, In Situ Size Measurements, and Individual Particle Tracking

X. Li, G. Salzano, J. Zhang, J. Zhang and R. Gref

Other Formulations

Supramolecular cyclodextrin-based nanoparticles (CD-NPs) mediated by host-guest interactions have gained increased popularity because of their “green” and simple preparation procedure, as well as their versatility in terms of inclusion of active molecules. Herein, we ...
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Publication - Abstract

Nov 01, 2016
Canadian Journal of Chemistry

Branched-Chain and Dendritic Lipids for Nanoparticles

M.W. Meanwell, C. O’Sullivan, P. Howard and T.M. Fyles

Other Formulations Oncology

Lipid nanoparticles (LNPs) for drug-delivery applications are largely derived from natural lipids. Synthetic lipids, particularly those incorporating branched hydrocarbons and hyper-branched hydrocarbon architectures, may afford enhanced lipophilic...
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Publication - Abstract

Sep 13, 2016
Chemical Society Reviews

Lipid-Based Nanovesicles for Nanomedicine

N. Grimaldi, F. Andrade, N. Segovia, L. Ferrer-Tasies, S. Sala, V. Veciana and N. Ventosa

Small Molecule Drugs Liposomes siRNA

Molecular self-assembly has enabled the fabrication of biologically inspired, advanced nanostructures as lipid-based nanovesicles (L-NVs). The oldest L-NVs, liposomes, have been widely proposed as potential candidates for drug delivery, diagnostic ...
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Publication - Abstract

May 12, 2016
Journal of Liposome Research

Micro-Spherical Cochleate Composites: Method Development for Monodispersed Cochleate System

L. Nagarsekar, M. Ashtikar, F. Steiniger, J. Thamm, F.H. Schacher and A. Fahr

Other Formulations

Cochleates have been of increasing interest in pharmaceutical research due to their extraordinary stability. However the existing techniques used in the production of cochleates still need significant improvements to achieve sufficiently monod...
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Publication - Abstract

Jul 13, 2015
Journal of the American Chemical Society

Rapid Synthesis of a Lipocationic Polyester Library via Ring-Opening Polymerization of Functional Valerolactones for Efficacious siRNA Delivery

J. Hao, P. Kos, K. Zhou, J.B. Miller, L. Xue, Y. Yan, H. Xiong. S. Elkassih and D.J. Siegwart

Oncology Other Formulations Other Applications

The ability to control chemical functionality is an exciting feature of modern polymer science that enables precise design of drug delivery systems. Ring-opening polymerization of functional monomers has emerged as a versatile method to prepare clinically translatable degradable ...
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Publication - Abstract

Apr 09, 2015
Journal of Liposome Research

Production of Limit Size Nanoliposomal Systems with Potential Utility as Ultra-Small Drug Delivery Agents

I.V. Zhigaltsev, Y.K. Tam, A.K. Leung and P.R. Cullis

mRNA Other Formulations Liposomes

Previous studies from this group have shown that limit size lipid-based systems—defined as the smallest achievable aggregates compatible with the packing properties of their molecular constituents—can be efficiently produced using a&nbs...
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Publication - Summary

Feb 21, 2012
Langmuir

Bottom-Up Design and Synthesis of Limit Size Lipid Nanoparticle Systems with Aqueous and Triglyceride Cores Using Millisecond Microfluidic Mixing

I.V. Zhigaltsev, N. Belliveau, I. Hafez, A.K. Leung, J. Huft, C. Hansen and P.R. Cullis

Liposomes Other Formulations Small Molecule Drugs

The potency of drug delivery systems can heavily rely on their ability to penetrate poorly vascularized tissues such as tumors, following intravenous administration. The drug delivery vehicle’s size greatly impacts this phenomenon.

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APPLICATION NOTES

Application Note

Sep 23, 2022

The Fundamentals of Developing mRNA-Based Therapeutics

Each regulatory phase of mRNA therapeutic development consists of specific goals, steps and end points. There are also some unique challenges and risks in each stage of the drug development process....
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Application Note

Dec 22, 2018

PLGA Microspheres Formulation and size-tuning using the NanoAssemblr® Benchtop

Polymeric Nanoparticles Other Formulations

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POSTERS

Poster

Feb 09, 2022

A Novel RNA Lipid Nanoparticle Platform: Gene-editing CAR T Cells for Off-the-Shelf Cancer Therapy

Nucleic Acid Lipid Nanoparticles Oncology Formulation

Download
Poster

Jun 11, 2018

Rapid development and seamless scale-up of genetic nanomedicines

mRNA Nucleic Acid Lipid Nanoparticles Genetic Medicine

Download

VIDEOS & WEBINARS

Video

Aug 10, 2017

SAM (Self Amplifying Message) for Rapid Response to Emerging Infectious Diseases - Derek O'Hagan

mRNA Vaccines Other Formulations

Derek O’Hagan, Global Head of Vaccine Chemistry and Formulation GSK vaccines Research in Cambridge | 2nd Annual Nanomedicines Symposium, July 15th, 2017
Video

Nov 30, 2016

"Nanoparticle Engineering by Microfluidics" - Ferdia Bates, BioNTech

Vaccines Liposomes mRNA

From our 2016 Nanomedicines Symposium in LONDON, UK NOVEMBER 29th, 2016
Video

Aug 10, 2016

NanoAssemblr® Benchtop | Dr. Gesine Heuck, PNI Tech Talk

siRNA Small Molecule Drugs Other Payloads

NanoAssemblr® Benchtop Tech Talk from 2016 Nanomedicine Symposium
Video

Jul 25, 2016

Lipid Particle Formation Procesess using Microfluidic Mixing| Ben Boyd, Monash University

Liposomes Other Formulations

in situ Interrogation of structured Lipid Particle Formation Processes using Microfludic Mixing Devices | Ben Boyd, Monash University
Video

Jul 26, 2016

Preparation of Nano-Droplets for Ultrasound Theranostics by using the NanoAssemblr® | Kazuo Maruyama

DNA Other Formulations Oncology

Nanomedicine Symposium

Preparation of nano droplets for ultrasound theranostics by using the NanoAssemblr® - Kazuo Maruyama - Teikyo University

Related Resources:
Video

Jul 25, 2016

Nanoparticle Size Characterization: Tips & Tricks for Light Scattering | Ulf Nobbmann, Malvern

Other Formulations Liposomes

Nanomedicine Symposium

Nanoparticle Size Characterization: Tips & Tricks for Light Scattering - Ulf Nobbmann - Malvern Instruments
Video

Sep 14, 2016

Nanomedicines: Materials, Manufacturing & Therapeutic Applications | James Taylor, CEO, PNI

Other Formulations Polymeric Nanoparticles Nucleic Acid Lipid Nanoparticles

2016 Nanomedicines: Materials, Manufacturing & Therapeutic Applications Symposium, July 16th in Seattle, WA

Introductory talk: James Taylor, CEO, Precision NanoSystems (PNI) - Nanomedicines: Materials, Manuf...
Video

Dec 15, 2016

"Facing the Challenge of Reproducible and Scalable Nanoparticle Production for RNA Delivery" - Ray Schiffelers, Keynote Speaker

Nucleic Acid Lipid Nanoparticles mRNA Small Molecule Drugs

Keynote Speaker from our Nanomedicines Symposium November 29th, London UK

Ray Schiffelers - Facing the Challenge of Reproducible and Scalable Nanoparticle Production for RNA Delivery - Keynote
Video

Nov 30, 2016

"Advanced Nanofibre and Liposome-Based Biocompatible Nanomaterials for Construction of Vaccines" - Jaroslav Turanek

DNA Other Formulations Liposomes

Nanomedicines Symposium

Jaroslav Turanek - Veterinary Research Institute, Brno Czech Republic -
Webinar

Apr 27, 2017

Scaling Nanoparticle Production from Bench to Clinic

Liposomes Nucleic Acid Lipid Nanoparticles Other Formulations

First webinar in a three part series.

Presented by Kevin Ou, Senior Manager of Pharmaceutical Operations at Precision NanoSystems Inc.
Webinar

Jun 21, 2016

Microfluidic Strategies to Improve Encapsulation of Hydrophobic Drugs in Nanoparticles

Other Formulations Small Molecule Drugs Polymeric Nanoparticles

Microfluidic-mediated self-assembly of liposomes and polymer nanoparticles expands the strategies available to the formulation scientist for the encapsulation and delivery of hydrophobic compounds. In this webinar Dr. James Taylor and Dr. Shyam Gar...

ARTICLES

Article

Dec 08, 2023

RNA-LNPs: Pioneering a Regulatory Path to Success

Navigating global regulations for RNA-LNP therapeutics with early regulatory strategy and by establishing LNP Manufacturing Partners
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Article

Dec 01, 2023

The Crucial Role of Analytics and Bioassays to Fast-Track mRNA-LNP Drug Development

Analytics and bioassays monitor variables like RNA, LNP composition, polydispersity, particle size, and manufacturing process to accelerate RNA-LNP production.
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Article

Nov 01, 2023

Outsourcing RNA-LNP Production: A Key to Competitive Success

Outsourcing RNA–LNP drug manufacturing accelerates drug development with advanced cGMP technologies, comprehensive analytical testing, and biopharma services.
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Article

Jun 05, 2023

Fast Track RNA-LNP Drug Development by Lowering the Lipid Barrier

Targeted Drug Delivery Other Formulations

Rapidly develop and optimize LNP-based drug delivery systems for clinical applications by accessing diverse range of pre-screened lipid compositions.
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Article

Jan 18, 2023

Fit-For-Purpose Lipids Can Accelerate, Scale and Improve RNA-LNP Drugs

Nucleic Acid Lipid Nanoparticles Formulation

Lipid Nanoparticles (LNPs) are nanoparticles formed by lipid components that are one of the effective means for mRNA delivery. Although the emergence of variants of COVID-19 has encouraged greater demands for developing mRNA–LNP vaccines, its benefits extend beyond vaccinations t...
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Article

Sep 28, 2022

Expand mRNA-LNP Nanomedicine Possibilities through Optimizing Lipid Nanoparticle Formulations

Genetic Medicine Nucleic Acid Lipid Nanoparticles

Nanomedicine offers promising therapeutic solutions for cell and gene therapies, autoimmune diseases, oncology, rare inherited diseases and more. Some new avenues to explore include early disease detection or molecularly tailored treatments for patients who have complex diseases ...
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